Robert E. Campbell
Department of Chemistry University of Alberta
Edmonton, Alberta T6G 2G2 CANADA
Office phone: (780) 492-1849
Lab phone: (780) 492-0229
Fax: (780) 492-8231
Email: robert.e.campbell@ualberta.ca
Web: www.chem.ualberta.ca/faculty/campbell.htm
Current Appointments
|
Assistant Professor and Canada Research Chair |
2003 to present: in Bioanalytical Chemistry
at the University of Alberta,
Department of Chemistry |
Education And Training
|
2000-2003 |
Postdoctoral fellow
|
University of California, San Diego
Department of Pharmacology |
|
1994-2000 |
Ph.D. |
University of British Columbia
Department of Chemistry |
|
1990-1994 |
B.Sc.
|
University of British Columbia
Department of Chemistry |
Awards
|
2008 |
Petro Canada Young Innovator Award |
|
2004-2009 |
Tier II Canada Research Chair in Bioanalytical Chemistry |
|
2004 |
Alberta Ingenuity New Faculty Award |
| 2002 |
Boehringer Ingelheim Award for Organic or Bioorganic Chemistry for doctoral research of outstanding quality. Presented at the 85th Canadian Society for Chemistry Conference, Vancouver, British Columbia |
| 2000 |
Bio Mega/Boehringer Ingelheim Scholarship for Organic Chemistry for excellence in graduate research |
Overview & Research Program
Dr. Campbell is focused on the invention and optimization of tools that allow researchers to peer at the inner workings of, live cells. Specifically, his group is dedicated to the development new intracellular biosensors based on one of the most important classes of proteins in biotechnology: fluorescent proteins encoded by genes cloned from marine organisms such as jellyfish and reef corals. These biosensors are used to detect the activity of enzymes in live cells and he is currently using them to help identify new inhibitors of enzymes that catalyze epigenetic modifications associated with cancer.
New fluorescent proteins originating from the Campbell lab have been licensed to biotechnology companies and others are freely distributed to any academic research group that requests them.
His accomplishments have most recently been acknowledged with an Alberta Ingenuity New Faculty Award and a Petro-Canada Young Innovator Award.
Recent Publications
(names of trainees in bold, * denotes corresponding author)
H-w. Ai, K.L. Hazelwood, M.W. Davidson, and R.E. Campbell*, "Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors", Nature Methods, 2008, 5, 401-403.
H-w. Ai, S.G. Olenych, P. Wong, M.W. Davidson, and R.E. Campbell*, "Live cell fluorescence imaging with hue-shifted monomeric variants of Clavularia cyan fluorescent protein", BMC Biology , 2008, 6 : 13. Designated Highly Accessed and Featured article.
H-w. Ai, and R. E. Campbell*, "Teal fluorescent proteins: Characterization of a reversibly photoconvertible variant", Proceedings of SPIE, 2008, 6868, 68680D.
Q.K. Timerghazin, H.J. Carlson, C. Liang, R.E. Campbell,* and A. Brown*, ‘Computational prediction of absorbance maxima for a structurally diverse series of engineered green fluorescent protein chromophores’, J. Phys. Chem B, 2008, 112: 2533-2541.
Y. Li, A.M. Sierra, H-w. Ai, and R.E. Campbell*, "Identification of sites within a monomeric red fluorescent protein that tolerate peptide insertion and testing of corresponding circular permutations", Photochemistry and Photobiology, 2008, 84: 111–119.
H-w. Ai and R.E. Campbell*, ‘More than just pretty colors: the growing impact of fluorescent proteins in the life sciences’, Biotechnology Focus , 2007, issue 11: 16-18. (invited review).
Z. Cheng, M. Miskolzie, and R. E. Campbell*, "In vivo screening identifies a highly folded beta-hairpin peptide with a structured extension", ChemBioChem, 2007, 8: 880-883. Featured with cover art.
H.-w. Ai , Nathan C. Shaner, Z. Cheng , R. Y. Tsien, and R. E. Campbell*, "Exploration of new chromophore structures leads to the identification of improved blue fluorescent proteins", Biochemistry , 2007, 46 : 5904 -5910. News story featured on the cover of June 2007 issue of Biophotonics. 9th Most-accessed article in Biochemistry for 2007.
J. N. Henderson, H.-w. Ai , R. E. Campbell, and S. J. Remington*, "Structural basis for reversible photobleaching of a green fluorescent protein homologue", Proc. Natl. Acad. Sci. U.S.A., 2007, 14: 6672-6677. News story in June 2007 issue of Biophotonics and April 2007 Science Daily online.
Z. Cheng and R. E. Campbell*, "Fluorescence-based characterization of genetically encoded peptides that fold in live cells: progress towards a generic hairpin scaffold", Proceedings of SPIE, 2007, 6449, 64490S.
H-w. Ai, J.N. Henderson, S.J. Remington, and R.E. Campbell*, "Directed evolution of a monomeric, bright, and photostable version of J.N. Henderson, S.J. Remington, and
R.E. Campbell*, "Directed evolution of a monomeric, bright, and photostable version of Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imaging", Biochem. J., 2006, 400: 531-540.
J.N. Henderson, S.J. Remington, and R.E. Campbell*, "Directed evolution of a monomeric, bright, and photostable version of
Z. Cheng and R. E. Campbell*, "Assessing the Structural Stability of Designed -Hairpin Peptides in the Cytoplasm of Live Cells", ChemBioChem, 2006, 7: 1147-1150.
R. E. Campbell*, "Realization of -lactamase as a versatile fluorogenic reporter", Trends Biotech, 2004, 22: 208-211.